I  U  P  A  C

 

 

 

News & Notices

Organizations & People

Standing Committees

Divisions

Projects

Reports

Publications
. . CI
. . PAC
. . Macro. Symp.

. . Books
. . Solubility Data

Symposia

AMP

Links of Interest

Search the Site

Home Page

 

Pure Appl. Chem. 76(4), 879-888, 2004

Pure and Applied Chemistry

Vol. 76, Issue 4

In situ poly(ethylene imine) coating of hollow fiber membranes used for microdialysis sampling

N. Torto, M. Ohlrogge, L. Gorton, J. M. Van Alstine, T. Laurell, and G. Marko-Varga

Department of Chemistry, University of Botswana, P/Bag UB 00704, Gaborone,
Botswana; Department of Analytical Chemistry, University of Lund, P.O. Box 124, SE-221 00, Lund, Sweden; Department of Chemical Engineering and Technology, KET/TS, Royal Institute of Technology, SE-100 44, Stockholm, Sweden; Department of Electrical Measurements, University of Lund, P.O. Box 118, SE-221 00, Lund, Sweden; Bioanalytical Research, Astra-Zeneca, Pre-Clinical Research and Development, P.O.Box 34, SE-221 00, Lund, Sweden

Abstract: A method for the in situ modification of hollow fiber membranes used as sampling units for microdialysis probes is presented. The method consists of adsorption-coating, high-
molecular-weight poly(ethylene imine), PEI, onto membranes, already fitted on microdialysis probes. Modification of membranes was designed to specifically explore the so-called Andrade effects and thus enhance the interaction of membranes with enzyme. The
performances of polysulfone, polyethersulfone, and polyamide membranes modified with PEI-enzyme complex were evaluated based on the membrane extraction fraction for maltose and maltotriose and membrane morphology as examined by scanning electron microscopy. Of the membranes tested, the PEI-enzyme complex least affected the performance of the polysulfone membranes. Conversion of maltoheptaose to maltotriose and maltose was increased reproducibly (within a 5 % relative standard deviation) by 50 % for modified membranes compared to the native hollow fiber membranes. The results demonstrate the potential to effectively modify membranes already fitted on a microdialysis probe. Such a
procedure can be modified and employed to either promote or reduce membrane–protein interaction for hollow fibers used as microdialysis sampling units or other similar membrane
applications.

*Plenary lectures presented at the Inaugural Conference for the Southern and Eastern Africa Network of Analytical Chemists (SEANAC), Gaborone, Botswana, 7-10 July 2003. Other presentations are published in this issue, pp. 697-888.


Page last modified 11 June 2004.
Copyright © 2004 International Union of Pure and Applied Chemistry.
Questions or comments about IUPAC, please contact, the Secretariat.
Questions regarding the website, please contact web manager.