Capillary electrophoretic techniques toward the metabolome analysis*
Shigeru Terabe1,**, Michal J. Markuszewski1, Naoko Inoue1, Koji Otsuka1,
and Takaaki Nishioka2
1Faculty of Science, Himeji Institute of Technology,
Kamigori, Hyogo, 678-1297 Japan; 2Graduate School of Agriculture, Kyoto
University, Sakyo-ku, Kyoto, 606-8502 Japan
Abstract: Metabolome analysis is a systematic chemical analysis
of metabolites, which may be used to investigate the metabolic activity
in the cell. Capillary electrophoresis (CE) is one of the most promising
techniques for the metabolome analysis, because it gives high-resolution
separations in a reasonable time and requires a minimum amount of samples.
General characteristics of CE are discussed from the viewpoint of metabolome
analysis. Micellar electrokinetic chromatography (MEKC), a separation
mode of CE, enables the separation of neutral analytes by using micelles
as pseudostationary phases. MEKC is also powerful for the separation
of ionic analytes to improve selectivity. To solve relatively poor concentration
sensitivity with UV absorbance detection, on-line sample preconcentration
techniques were developed resulting in up to few thousand-fold increases
in sensitivity. Laser-induced fluorescence detection is another solution
to increase concentration sensitivity, but most analytes are not natively
fluorescent. Therefore, several derivatization reactions were performed
to selectively detect a class of analytes with high sensitivity. Some
preliminary results are shown with formic acid extracts of Bacillus
*Lecture presented at the IUPAC International Congress
on Analytical Sciences 2001 (ICAS2001), Tokyo, Japan, 6-10 August 2001.
Other presentations are published in this issue,
** Corresponding author
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