I  U  P  A  C

News & Notices

Organizations & People

Standing Committees




. . CI
. . PAC
. . Macro. Symp.

. . Books
. . Solubility Data



Links of Interest

Search the Site

Home Page


Pure Appl. Chem. Vol. 73, No. 10, pp. 1563-1572 (2001)

Pure and Applied Chemistry

Vol. 73, Issue 10


Capillary electrophoretic techniques toward the metabolome analysis*

Shigeru Terabe1,**, Michal J. Markuszewski1, Naoko Inoue1, Koji Otsuka1, and Takaaki Nishioka2

1Faculty of Science, Himeji Institute of Technology, Kamigori, Hyogo, 678-1297 Japan; 2Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502 Japan

Abstract: Metabolome analysis is a systematic chemical analysis of metabolites, which may be used to investigate the metabolic activity in the cell. Capillary electrophoresis (CE) is one of the most promising techniques for the metabolome analysis, because it gives high-resolution separations in a reasonable time and requires a minimum amount of samples. General characteristics of CE are discussed from the viewpoint of metabolome analysis. Micellar electrokinetic chromatography (MEKC), a separation mode of CE, enables the separation of neutral analytes by using micelles as pseudostationary phases. MEKC is also powerful for the separation of ionic analytes to improve selectivity. To solve relatively poor concentration sensitivity with UV absorbance detection, on-line sample preconcentration techniques were developed resulting in up to few thousand-fold increases in sensitivity. Laser-induced fluorescence detection is another solution to increase concentration sensitivity, but most analytes are not natively fluorescent. Therefore, several derivatization reactions were performed to selectively detect a class of analytes with high sensitivity. Some preliminary results are shown with formic acid extracts of Bacillus subtilis.

*Lecture presented at the IUPAC International Congress on Analytical Sciences 2001 (ICAS2001), Tokyo, Japan, 6-10 August 2001. Other presentations are published in this issue, pp. 1555-1623.

** Corresponding author

Back to Contents for access to full text


Page last modified 11 January 2002.
Copyright © 2002 International Union of Pure and Applied Chemistry.
Questions or comments about IUPAC, please contact, the Secretariat.
Questions regarding the website, please contact web manager.