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Pure Appl. Chem. Vol. 73, No. 6, pp. 969-992 (2001)

Pure and Applied Chemistry

Vol. 73, Issue 6


Retention Parameters in Chromatography (IUPAC Recommendations 2001)

Part A. Hold-up Volume Concept in Column Chromatography

José Antonio García Domínguez1 and José Carlos Díez-Masa2

1Institute of Physical Chemistry "Rocasolano", CSIC, Serrano, 119. 28006 Madrid, Spain; 2Institute of Organic Chemistry, CSIC, Juan de la Cierva, 3. 28006 Madrid, Spain

Part B. Retention parameters in gas chromatograpy

Vadim A. Davankov

Nesmeyanov-Institute of Organo-Element Compounds, Russian Academy of Sciences, Moscow, 119991, Vavilov Str. 28, Russia

Abstract: The paper presents a revision of terms in the IUPAC "Nomenclature for Chromatography", Pure and Applied Chemistry, 65, 819-872, 1993. The terms revised pertain to hold-up volumes in gas, liquid, and supercritical-fluid chromatography, as well as to basic retention parameters, especially in gas chromatography. A number of related and derived definitions are described, including definitions of the terms "chromatographic process" and "chromatographic phase system". A number of the original terms were found to be misleading or superfluous, including such terms as corrected retention time, net retention time, total retention volume (time), and specific retention volume at 0 °C, and their use is strongly discouraged

In Part A, the concept of the hold-up volume in chromatography is discussed. The paper also compares methods described in the literature to determine the hold-up volume.

In Part B, retention parameters in gas chromatography are discussed with the aim of (i) emphasizing the physical meaning of the terms and (ii) specifying the temperatures and pressures for the terms for gas volumes and flow rates.

The appendix presents revised recommendations for the terminology of some items, as well as those that are not recommended.

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