Looking at the photodynamics of individual fluorescent molecules
M. F. García-Parajó**, J.-A. Veerman, L. Kuipers, and
N. F. van Hulst
Applied Optics Group, Faculty of Applied Physics, MESA+
Research Institute, University of Twente, P.O. Box 217, 7500 AE Enschede,
Abstract: The photodynamics of individual molecules and fluorescent
proteins has been investigated in real time. In the case of organic
molecules, both the triplet state lifetime and intersystem crossing
yield appear to vary in time and space. In the case of autofluorescent
proteins, light-driven "on-off" fluorescence emission has
been observed, with a behavior that is consistent with the existence
of a long-lived dark state.
*Lecture presented at the XVIIIth IUPAC Symposium on
Photochemistry, Dresden, German , 22-27 July 2000.Other presentations
are published in this issue, pp.395-548.
** Corresponding author