35 No. 4
||Recent IUPAC technical reports and recommendations that affect the many fields of pure and applied chemistry.
See also www.iupac.org/publications/pac
Pure and Applied Chemistry, 2013
Vol. 85, No. 5, pp. 999–1016
This report is an overview on the applicability of fluorescence correlation spectroscopy (FCS) for the accurate determination of translational diffusion coefficients and thus, via the Stokes–Einstein relation, of molecular size. Several of the most common sources of optical aberrations and their impact on the outcome of conventional FCS measurements were considered. A new variant of FCS, dual-focus FCS, which is robust against most of the considered aberrations is also described and reference values of diffusion coefficients for several fluorescent dyes across the visible spectrum are reported.
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