||I U P A C
Organizations & People
and Biophysical Chemistry Division (I)
Procedures for the measurement of the interparticle
distance dependence of colloidal force data using the atomic force
microscope colloid probe technique
Methods by which the force-distance data can be
compared with existing models of interparticle forces.
The colloid probe AFM technique is a promising new method to
determine the interactions between colloidal bodies. The technique
is based on the general AFM and this equipment is, in principle, available
for many research groups (not excessively expensive). In order to
avoid confusion in the literature about the use of the AFM equipment
for colloid stability measurements (and consequently about the interpretation
of results) recommendations for the use of this equipment for this
type of measurement are needed.
Principles of atomic force microscopy
Attachment of colloidal probe to cantilever
Determination of spring constant
Determination of colloid probe radius
Non-deformable and deformable surfaces
Symmetric and asymmetric interactions
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